Exercise #2:
Short peptides: Database search and multiple alignment
Several proteins were identified in the supernatant of a bacterial cell culture.
One of these proteins was isolated and the N-terminal amino acid sequence of three fragments was determined by Edman degradation.
The following peptide sequences were obtained:
| Peptide |
Sequence |
| Peptide #1 |
ALSGANGQM |
| Peptide #2 |
SLLGDMSASDEA |
| Peptide #3 |
KSMNNKIT |
- From which protein could the peptides derive?
- Find all homologous proteins in the sequence database!
- Construct a multiple sequence alignment of this protein family! Is there anything special?
Latest update: October 15, 2009
Ralf Koebnik
Institut de recherche pour le dèveloppement
UMR 5096, CNRS-UP-IRD
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